需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
5,722 Views
•
08:22 min
•
December 16, 2021
DOI:
Summary
Automatically generated
Here we outline and demonstrate a protocol for primary mouse liver sinusoidal endothelial cell (LSEC) isolation. The protocol is based on liver collagenase perfusion, nonparenchymal cell purification by low-speed centrifugation, and CD146 magnetic bead selection. We also phenotype and characterize these isolated LSECs using flow cytometry and scanning electron microscopy.
Read Article
Cite this Article
Guo, Q., Furuta, K., Aly, A., Ibrahim, S. H. Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells. J. Vis. Exp. (178), e63062, doi:10.3791/63062 (2021).
Copy