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Biochemistry

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JoVE Journal Biochemistry
Påvisning og kvantifisering av kalsitoningenrelatert peptid (CGRP) i humant plasma ved bruk av en modifisert enzymbundet immunosorbentanalyse
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Journal (Biochemistry)
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Journal (Biochemistry)
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
 
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Påvisning og kvantifisering av kalsitoningenrelatert peptid (CGRP) i humant plasma ved bruk av en modifisert enzymbundet immunosorbentanalyse

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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Publiserte data vedrørende kalsitoningenrelaterte peptidkonsentrasjoner (CGRP) i humant plasma er inkonsistente. Disse inkonsekvensene kan skyldes mangelen på en standardisert, validert metodikk for å kvantifisere dette nevropeptidet. Her beskriver vi en validert ELISA-protokoll (enzyme-linked immunosorbent assay) for å rense og kvantifisere CGRP i humant plasma.

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