Chongqing Academy of Chinese Materia Medica 3 articles published in JoVE Medicine Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development Jiuyu Fan1, Xingjian Wen1,2, Shengrong Li1, Rui Chu1, Yilong Chen1, Zhimin Su1, Na Li1,2 1Chongqing Academy of Chinese Materia Medica, 2Bioengineering College of Chongqing University In this protocol, the mutton-oil processing technology of Epimedii folium (EF) was optimized by applying a Box-Behnken experimental design-response surface methodology, and the effect of crude and optimized water-extracted EF on zebrafish embryonic development was preliminarily investigated. Medicine Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea Yilong Chen1, Na Li1, Dan Wang1, Jiuyu Fan1, Rui Chu1, Shengrong Li1 1Chongqing Engineering Research Center of Pharmaceutical Evaluation of Chinese Medicine, Chongqing Academy of Chinese Materia Medica Here, a comparative analysis of raw and processed Cyperi rhizoma (CR) samples is presented using ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UPLC-MS/MS) in rats with primary dysmenorrhea. The changes in blood levels of the metabolites and the sample constituents were examined between rats treated with CR and CR processed with vinegar (CRV). Medicine Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model Xingjian Wen*1,2, Jia Wang*1, Jiuyu Fan1, Rui Chu1, Yilong Chen1, Yajing Xing1, Na Li1,2, Guixue Wang2 1Chongqing Academy of Chinese Materia Medica, 2Bioengineering College of Chongqing University This protocol investigates the protective effects of platycodin D on non-alcoholic fatty liver disease in a palmitic acid-induced in vitro model.