North-Western State Medical University 1 article published in JoVE Developmental Biology Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors Artem Eremeev1,3, Lilia Belikova1,2, Evgeny Ruchko1, Egor Volovikov1,5, Olga Zubkova1, Alexy Emelin4, Roman Deev4, Olga Lebedeva1,3, Alexandra Bogomazova1,3, Maria Lagarkova1,3 1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.
