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Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Article DOI: 10.3791/62987-v • 10:16 min • December 11th, 2021
December 11th, 2021 •
1,3, 1,2, 1, 1,5, 1, 4, 4, 1,3, 1,3, 1,3
1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University

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Summary
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Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.

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Brain Organoid Generation Induced Pluripotent Stem Cells Mini Bioreactors Cerebral Organoids 3D Model Central Nervous System Pathologies Pharmacologically-active Substances Regenerative Medicine Protocol Differentiation Cultivation Shaker Low-adhesion Devices Market Tubes Plates Bioreactors Nutrient Medium Size And Morphology Scaling The Process Sterile Centrifuge Tubes
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