Chang Gung University 11 articles published in JoVE Medicine Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium Chun Hsiung*1,2, Yu-Ting Liu*1,2, Chen-Ying Su3, Chung-Chuan Hsiung4,5, Kuo-Hsuan Hung2,6,7, Lung-Kun Yeh2,6 1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo. Neuroscience Transtubular Endoscopic Posterolateral Decompression for L5-S1 Lumbar Lateral Disc Herniation Masato Tanaka1, Shinya Arataki1, Rahul Mehta1, Tsung-Ting Tsai2, Yoshihiro Fujiwara1, Koji Uotani1, Taro Yamauchi1 1Department of Orthopedic Surgery, Okayama Rosai Hospital, 2Department of Orthopedic Surgery, Chang Gung University Presented here is a novel technique of C-arm free transtubular posterolateral decompression for lumbar foraminal stenosis and lateral disc herniation under O-arm navigation. Bioengineering Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices Hui-Fang Chang1, Shih-En Chou1, Ji-Yen Cheng1,2,3,4 1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang Ming Chao Tung University, 3Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 4College of Engineering, Chang Gung University In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system. Cancer Research Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres Chun-Chia Cheng1, Po-Jui Hsu4, Zong-Lin Sie1, Fang-Hsin Chen1,2,3 1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells. Medicine Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand Yi-Mei Chen1, Szu-Shen Lai1, Yu-Cheng Pei2,3,4,5, Chia-Ju Hsieh1, Wei-Han Chang1 1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, 2Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, 3School of Medicine, Chang Gung University, 4Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, 5Healthy Aging Research Center, Chang Gung University This study reports the development of a novel robot-assisted task-oriented program for hand rehabilitation. The developmental process consists of experiments using both healthy subjects and subjects who have had a stroke and suffered from subsequent motor control dysfunction. Bioengineering A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva Yueh-Feng Wu1, Rai-Teng Ye1, Ming-Kai Pan2,3, Sung-Jan Lin*1,3,4,5, Hsin-Yuan Tan*6,7 1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures. Medicine Integration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure Shu-Chun Huang1,2,3, Carl PC Chen1,3, Tieh-Cheng Fu4, Yu-Jen Chen1,5,6 1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, 2Healthy Aging Research Center, Chang Gung University, 3School of Medicine, College of Medicine, Chang Gung University, 4Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Keelung, 5National Quemoy University, 6Kinmen Hospital, Ministry of Health and Welfare This protocol integrated near-infrared spectroscopy into conventional cardiopulmonary exercise testing to identify the involvement of the cerebral hemodynamic response in exercise intolerance in patients with heart failure. Neuroscience Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release Ya-Tin Lin1, Jin-Chung Chen1,2,3 1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist. Engineering One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes Chien-Kai Wang1,2, Wei-Hao Liao1, Hsiao-Mei Wu1, Yi-Chung Tung1,3 1Research Center for Applied Sciences, Academia Sinica, 2Department of Civil Engineering, Tamkang University, 3College of Engineering, Chang Gung University Several methods are available for the fabrication of channels of non-rectangular sections embedded in polydimethylsiloxane microfluidic devices. Most of them involve multistep manufacturing and extensive alignment. In this paper, a one-step approach is reported for fabricating microfluidic channels of different geometric cross sections by polydimethylsiloxane sequential wet etching. Bioengineering Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells Yen-Li Wang1,2, Adrienne Hong3, Tzung-Hai Yen1,4,5,6, Hsiang-Hsi Hong1,7,8 1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes. Neuroscience A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood Chia-Hsiang Chen1,2, Yu-Syuan Chang1 1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
