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Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
JoVE Journal
Medicine
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JoVE Journal Medicine
Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
DOI:

03:55 min

March 24, 2023

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Chapters

  • 00:04Introduction
  • 01:07Preparation for the Modified Autologous Conditioned Serum (mACS) Drops
  • 02:15Results: Assessment of Murine Corneal Wound Healing
  • 03:03Conclusion

Summary

Automatic Translation

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

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