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Journal / Bioengineering / Systematic Analysis of In Vitro Cell Rolling…
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Bioengineering

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Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Article DOI: 10.3791/50866-v • 11:04 min • October 16th, 2013
October 16th, 2013 •
1,2,3,4,5, 1,2,3,4,5, 1,2,3,4,5, 6, 1,2,3,4,5
1Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, 2Center for Regenerative Therapeutics, Brigham and Women's Hospital, 3Harvard Medical School, Harvard University, 4Harvard Stem Cell Institute, Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering, Massachusetts Institute of Technology

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Summary
Automatic Translation

This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.

Tags

In Vitro Cell Rolling Multi-well Plate Microfluidic System Cell-based Therapy Cell Homing Vascular Endothelium Parallel Plate Flow Chamber (PPFC) Multi-parameter Software Analysis Platform Rolling Velocities Rolling Path HL-60 Cells P-selectin E-selectin Tumor Necrosis Factor-alpha (TNF-alpha) Endothelial Cells (ECs)
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