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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Article DOI: 10.3791/51138-v • 06:30 min • February 5th, 2014
February 5th, 2014 •
1,2,3, 1, 1, 4, 1,2,3,5
1Division of Pediatric Neurology, Department of Pediatrics, University of Utah School of Medicine, 2Department of Neurobiology and Anatomy, University of Utah School of Medicine, 3Interdepartmental Program in Neurosciences, University of Utah School of Medicine, 4Mutation Generation and Detection Core, HSC Core Research Facility, University of Utah School of Medicine, 5Department of Neurology, University of Utah School of Medicine

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Summary
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PCR combined with high-resolution melt analysis (HRMA) is demonstrated as a rapid and efficient method to genotype zebrafish.

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Zebrafish Genotyping PCR High-resolution Melt Analysis Vertebrate Model System Development Study Disease Modeling Drug Screening Genetic Tools Mutations Transgenic Lines Large-scale Screening Genotyping Methods Time-consuming Labor-intensive Rapid Technique Sensitive Analysis Inexpensive Approach Contamination Artifacts High-throughput Screening Protocols
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