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Bioengineering

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JoVE Journal Bioengineering
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Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Article DOI: 10.3791/53205-v • 12:31 min • December 8th, 2015
December 8th, 2015 •
*1, *1, 1
1Center for Alzheimer's and Neurodegenerative Diseases, University of Texas Southwestern Medical Center
* These authors contributed equally

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Summary
Automatic Translation

Cell-to-cell transfer of protein aggregates, or proteopathic seeds, may underlie the progression of pathology in neurodegenerative diseases. Here, a novel FRET flow cytometry assay is described that enables specific and sensitive detection of seeding activity from recombinant or biological samples.

Tags

Sensitive Detection Proteopathic Seeding Activity FRET Flow Cytometry Transcellular Propagation Toxic Protein Aggregates Neurodegenerative Diseases Alzheimer's Disease Tauopathies Seeding Assay Fluorescence Resonance Energy Transfer (FRET) Flow Cytometry Monoclonal Cell Line Ultra-sensitive Recombinant Samples Biological Samples Human Brain Homogenates Mouse Brain Homogenates Monoclonal HEK 293T Cells Aggregation-prone Repeat Domain (RD) P301S Mutation CFP YFP FRET Signal Protein Aggregation Biosensor Cells Uptake Of Tau Seeds Aggregation-induced FRET Femtomolar Concentrations
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