Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Priyanka Dutta; Christina Lehmann; Devang Odedra; Deepika Singh; Christian Pohl

doi:10.3791/53469

Journal

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Biologie

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Tracking und Quantifizierung von Entwicklungsprozessen in C. elegans Verwendung von Open-Source-Werkzeuge

Tracking and Quantifying Developmental Processes in <em>C. elegans</em> Using Open-source Tools

JoVE Journal
Biologie

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JoVE Journal Biologie

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Tracking und Quantifizierung von Entwicklungsprozessen in C. elegans Verwendung von Open-Source-Werkzeuge

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8,846 Views

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10:41 min

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December 16, 2015

DOI:

10.3791/53469-v

DOI:

10.3791/53469-v

•

10:41 min

December 16, 2015

•

8841 Views

Priyanka Dutta*¹, Christina Lehmann*¹, Devang Odedra*¹, Deepika Singh*¹, Christian Pohl¹

¹Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine,Goethe University

Summary

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.