JoVE Journal
Biology
Biology
Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.
Kapitel
Summary
We describe here a system utilizing a site-specific, reversible in vivo protein block to stall and collapse replication forks in Escherichia coli. The establishment of the replication block is evaluated by fluorescence microscopy and neutral-neutral 2-dimensional agarose gel electrophoresis is used to visualize replication intermediates.