Journal
/
Biologie
/
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
JoVE Journal
Biologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biologie
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

DOI:
10.3791/55215-v

•

08:04 min

•

March 12, 2017

•

, ,
1Institut Curie, INSERM U1196,Campus Universitaire d’Orsay, 2Institut Pasteur, Trypanosome Cell Biology Unit, Department of Parasites & Insect Vectors,INSERM U1201

Kapitel

  • 00:05Titel
  • 00:44Sample Preparation and Sectioning
  • 04:14Sample Imaging and Image Processing
  • 06:30Results: Through-focal Imaging of Trypanosoma brucei
  • 07:22Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.

Tags

Scanning Transmission Electron TomographyThick Biological Samples3D ReconstructionStructural BiologyCell Culture SampleParaformaldehydeGluteraldehydeOsmium TetroxideUranyl AcetateEthanolEpoxy ResinPolymerizationSpecimen Holder

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Miniaturized Sample Preparation for Transmission Electron Microscopy

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Read Article