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Journal / Biochemistry / Method for Efficient Refolding and Purification…
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Biochemistry

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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Article DOI: 10.3791/57092-v • 14:25 min • December 12th, 2017
December 12th, 2017 •
1,2, 1,2,3
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University

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Summary
Automatic Translation

A procedure is presented for the refolding of the dCACHE periplasmic ligand binding domain of Campylobacter jejuni chemoreceptor Tlp3 from inclusion bodies and the purification to yield milligram quantities of protein.

Tags

Refolding Purification Chemoreceptor Ligand Binding Domain Inclusion Bodies Structural Studies Functional Studies Ligand Screening Small Molecule Libraries Protein Recovery E.coli Expression Milligrams Of Protein LB Broth Ampicillin BL21-CodonPlus RIPL Cells PET151 D-TOPO Vector Hexahistidine TIp3-LBD Expression Erlenmeyer Flasks OD600 Measurement IPTG Induction Cell Harvest Centrifugation
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