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A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

Article DOI: 10.3791/57523-v • 09:21 min • October 11th, 2018
October 11th, 2018 •
1, 2, 1, 1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 2University of Bristol

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Summary
Automatic Translation

Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.

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Ligated Intestinal Loop Model Specific Pathogen Free Chickens Clostridium Perfringens Virulence Chicken Necrotic Enteritis Research Virulence Comparison Reduction Of Experimental Chickens Intestinal Pathogens Surgical Procedures Suturing Techniques Anesthesizing Vital Measurements Feather Removal Surgical Site Preparation
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