Dynamische Proteomic und MiRNA Analyse des Polysomes von isolierten Maus Herz nach Langendorff Perfusion
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August 29, 2018
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Hier präsentieren wir ein Protokoll zur Polysome Profilierung auf das Herz isoliert PERFUNDIERTEN Maus durchführen. Wir beschreiben Methoden für Herz Perfusion, Polysome Profilierung und Analyse der Polysome Fraktionen in Bezug auf mRNAs, MiRNAs und Polysome Proteom.
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Stastna, M., Thomas, A., Germano, J., Pourpirali, S., Van Eyk, J. E., Gottlieb, R. A. Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion. J. Vis. Exp. (138), e58079, doi:10.3791/58079 (2018).
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