JoVE Journal
Bioengineering
Bioengineering
Dieser Inhalt ist Open Access.
Kapitel
Summary
A detailed protocol for the purification and subsequent analysis of a monoclonal antibody from harvested cell culture fluid (HCCF) of automated microbioreactors has been described. Use of analytics to determine critical quality attributes (CQAs) and maximizing limited sample volume to extract vital information is also presented.