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Journal / Immunology and Infection / 分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
Journal (Immunology and Infection)
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Journal (Immunology and Infection)
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
 
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分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化

Article DOI: 10.3791/60541-v • 08:08 min • November 1st, 2019
November 1st, 2019 •
1,2, 1,2, 3, 1,2,3
1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen

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Summary
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这里介绍的是一种使用显微镜和荧光染色检测活细胞培养中巨噬细胞外陷阱 （MET） 生产的协议。此方案可进一步扩展，通过免疫荧光染色检查特定的 MET 蛋白标记物。

Tags

免疫学和感染，第153期，细胞外陷阱，巨噬细胞，MET，炎症，SYTOX绿色，荧光显微镜
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