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Journal / Biochemistry / 使用开源 smfBox 进行精确和准确的单分子 FRET…
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Biochemistry

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JoVE Journal Biochemistry
使用开源 smfBox 进行精确和准确的单分子 FRET 测量
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使用开源 smfBox 进行精确和准确的单分子 FRET 测量

Article DOI: 10.3791/62378-v • 07:12 min • July 5th, 2021
July 5th, 2021 •
*1, *1, 1, 1
1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
* These authors contributed equally

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本文提供了使用开源、廉价的 smfBox 对单个、自由扩散的生物分子进行完全校正的准确 FRET 测量的分步说明，从打开、通过对准和聚焦，再到数据收集和分析。

Tags

生物化学，第173期，单分子，FRET，显微镜，荧光，DNA，生物分子构象，共聚焦，结构测定，动力学
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