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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Article DOI: 10.3791/63973-v • 08:56 min • July 12th, 2022
July 12th, 2022 •
1, 1, 1, 1, 2, 1,2,3, 1
1Johannes Kepler University Linz, 2Charité - University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

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Summary
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Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.

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Simultaneous Imaging Flow-cytometry Detection Multiple Fluorescent Senescence Markers Therapy-induced Senescent Cancer Cells High Resolution Images Spatial Distribution Quantification Fluorescent Signals Cells Advanced Imaging Flow Cytometry System Speed Sensitivity Single Cell Images Spatial Information Flows Optometry Microscopy Data Acquisition DLBCL Cell Lines EdU Solution Carbon Dioxide Incubator Chloroquine Solution C12 FDG Solutions 2-phenylethyl-b
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