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Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

Article DOI: 10.3791/907-v • 10:00 min • August 14th, 2008
August 14th, 2008 •
1, 1, 2, 1, 1
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Neurobiology and Behavior, University of California, Irvine (UCI)

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Here we demonstrate a method for inducing and recording the progress of a delayed type-hypersensitivity (DTH) reaction in the rat ear. This is followed by a demonstration of the preparation of rat ear tissue for two-photon imaging of the effector / memory T cell response.

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Imaging Effector Memory T Cells Ear Induction Adoptive DTH Delayed Type Hypersensitivity Immune Reaction CCR7- T Lymphocytes Method Rat Ear Two-photon Imaging GFP-labeled Ova-specific Cell Line Equilibrate Challenge Saline Ova Conjugated To Texas-Red Antigen-presenting Cells Tissue Preparation Motility Of Cells Deep Dermal Layer Immune Response Collagen Fibers Second Harmonic Generation
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