Universidad de Sevilla 2 articles published in JoVE Neuroscience Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma Carlos Folgado-Dorado*1, Julia Caracena-De La Corte*1, José Raúl Aguilera-Velázquez1, Rafabel Santana-Villalona1, Alberto Rivera-Ramos1,2, Maria Pilar Carbonero-Aguilar1, Rocío Talaverón1,2, Juan Bautista1, Manuel Sarmiento Soto1,2 1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells. Medicine Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle Camille M. Selvais1, Laura L. De Cock1, Sonia M. Brichard1, María A. Davis-López de Carrizosa1,2 1Endocrinology, Diabetes and Nutrition Unit, Institute of Experimental and Clinical Research, Medical Sector, Université Catholique de Louvain, 2Departamento de Fisiología, Facultad de Biología, Universidad de Sevilla Increasing evidence indicates that excessive infiltration of lipids inside skeletal muscle results in lipotoxicity and diabetes. Here, we present a complete protocol, including tissue processing, staining with Bodipy, image acquisition, and analysis, to quantify the size, density, and subcellular distribution of lipid droplets in a fiber-type specific manner.
