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Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
JoVE Journal
Medicine
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JoVE Journal Medicine
Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
DOI:

11:50 min

June 08, 2022

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Chapters

  • 00:05Introduction
  • 01:06Cryosectioning, Fiber Typing, and Bodipy Staining
  • 04:06Acquisition of Images
  • 06:12Analysis of Images
  • 09:26Results: Fiber Type and Subcellular-Specific Quantification of Lipid Droplet
  • 10:55Conclusion

Summary

Automatic Translation

Increasing evidence indicates that excessive infiltration of lipids inside skeletal muscle results in lipotoxicity and diabetes. Here, we present a complete protocol, including tissue processing, staining with Bodipy, image acquisition, and analysis, to quantify the size, density, and subcellular distribution of lipid droplets in a fiber-type specific manner.

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