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Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Article DOI: 10.3791/1343-v • 08:36 min • May 26th, 2009
May 26th, 2009 •
1, 1, 1, 1, 1, 1
1Department of Surgery, University of Illinois, Chicago

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Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method.

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Human Pancreatic Islet Isolation Purification Culture Type 1 Diabetes Glucose Control Insulin Therapy Islet Transplantation Donor Characteristics Organ Procurement Preservation Isolation Outcome UIC (University Of Illinois At Chicago) Isolation Protocol Purification Gradient Cold Preservation Solutions (UW HTK) Cell Isolation Laboratory UIC Method Ricordi Et Al Method Digestion And Collection Of Pancreatic Tissue
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