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Immunology and Infection

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In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Article DOI: 10.3791/2390-v • 12:36 min • October 19th, 2010
October 19th, 2010 •
1, 2, 2, 1
1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University

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Resumen
Automatic Translation

This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.

Tags

In Vitro TRNA Methylation Assay Entamoeba Histolytica DNA Methyltransferase Dnmt2 Ehmeth Enzyme Protozoan Parasites Amebiasis Infectious Agents Clinical Manifestations 5-methylcytosine (5mC) Single DNA Methyltransferase Control Of Repetitive Elements Dual DNA/tRNAAsp Methyltransferase Activity Functional Significance TRNA Methyltransferase Activity Dnmt2 Proteins
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