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Journal / Biology / Single Cell Fate Mapping in Zebrafish
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Single Cell Fate Mapping in Zebrafish

Article DOI: 10.3791/3172-v • 07:53 min • October 5th, 2011
October 5th, 2011 •
1, 1, 1,2
1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 2Division of Hematology/Oncology, Cincinnati Children's Hospital Medical Center

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A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.

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Single Cell Fate Mapping Zebrafish Developmental Biology Fate Mapping Lineage Tracing Undifferentiated Precursor Cells Photoactivatable Proteins Fluorescence Spectrum UV Excitation Cell Tracing Probes Long-term Cell Tracking Caged Fluorescein-dextran Embryo Model Systems Uncaging Fluorescein-dextran Spatial Resolution Photoactivation Near-infrared Laser Pulses Titanium Sapphire Femtosecond Laser Two-photon Confocal Microscopes
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