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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Article DOI: 10.3791/53564-v • 09:49 min • April 6th, 2016
April 6th, 2016 •
1, 1, 1
1Biological Sciences, Virginia Tech

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A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.

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Enzymatic Processing Proteins MS Detection Flow-through Microreactor Fabrication Fast Digestion Protein Identification Electrospray Ionization Mass Spectrometer Amino Acid Sequence Structure And Function Characterization Fast And Cost-effective Protocol Microfabricated Platforms Glass Capillary Cleaver Clean Cut Glue Curing Electrospray Ionization Emitter PEEK Tube PEEK Union
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