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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Article DOI: 10.3791/53886-v • 09:20 min • March 31st, 2016
March 31st, 2016 •
1,2, 2, 2, 2, 2, 3, 1,2
1Institute of Biotechnology, National Taiwan University, 2Department of Animal Science and Technology, National Taiwan University, 3Department of Surgery, National Taiwan University Hospital and College of Medicine

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This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

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Adipose-derived Stem Cells Porcine Subcutaneous Adipose Tissues Differentiation Mesenchymal Adipocytes Osteocytes Chondrocytes Obesity Research Molecular Mechanism Adipose Tissue Development Stromovascular Cells Adipocyte Differentiation Biology Stem Cell Therapy Research Transdifferentiation Piglet Surgical Table Povidone Iodine Ethanol-soaked Gauze Pads Incision Fat Layer
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