Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / High-sensitivity Detection of Micrometastases…
JoVE Journal
Cancer Research

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Cancer Research
High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates
Journal (Cancer Research)
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
Journal (Cancer Research)
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
 

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

Capítulos

Resumen
Automatic Translation

To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.

Tags

High-sensitivity Detection Micrometastases GFP Lentivirus-transduced Organoids Patient-derived Colon Tumor Tumor Biology Colon Cancer Technique Detection GFP-labeled Mice Colon Tumor Organoid-derived Micrometastases Human Colorectal Cancer Cell Organoids Artificial Extracellular Matrix Patient-derived Tumor Xenograft Cell Pellet Colorectal Cancer Organoid Culture Medium Fetal Calf Serum (FCS) Cell Culture Incubator
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter