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In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Article DOI: 10.3791/59831-v • 07:10 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 2, 2, 1,3, 1
1Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, 3Nanosystems Initiative Munich

Capítulos

Resumen
Automatic Translation

Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.

Tags

Vesiculo Synthesis Peptide Membrane Precursors Autonomous Vesicle Growth Film Re-hydration Reaction Compartments Peptides Protocol Simplicity Robustness Sensitive Samples Centrifugal Vacuum Concentrator ELP Solution Chloroform Methanol Mixture Vortex Spherical Glass Beads Round Bottom Flask Rotary Evaporator Aluminum Foil Desiccator
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