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Biochemistry

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JoVE Journal Biochemistry
High-throughput proteinkrystallisering via mikrodialyse
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High-throughput proteinkrystallisering via mikrodialyse

Article DOI: 10.3791/64744-v • 06:18 min • March 3rd, 2023
March 3rd, 2023 •
1, 1, 2, 2, 1
1National Physical Laboratory, 2SWISSCI LTD

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Den præsenterede protokol beskriver en ligetil tilgang til screening af proteinkrystalliseringsbetingelser og krystalvækst ved hjælp af en 96-brønds dialyseplade med høj kapacitet. Anvendelsen af dialysatorrør til storskala vækst af mikrokrystaller er også demonstreret til seriel krystallografi og MicroED-applikationer.

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Retraktion udgave 193 proteinkrystallisation membranproteiner dialyse seriel krystallografi mikrodialyseplade dialysator
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