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Ekstracellulære Protein Microarray teknologi til High Throughput påvisning af lav affinitet Receptor-Ligand interaktioner
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
DOI:

06:01 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:28Extracellular Protein Microarray Printing
  • 01:55Preparation of Multivalent Bait Complexes
  • 03:09Extracellular Protein Microarray Screening
  • 04:36Results: Evaluation of Extracellular Protein Microarray Slide Printing and Screen of an Orphan Query Protein
  • 05:11Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol til skærmen ekstracellulære protein microarrays for identifikation af roman receptor-ligand interaktioner i høj overførselshastighed. Vi beskriver også en metode til at forbedre afsløring af forbigående protein-protein interaktioner ved hjælp af protein-microbead komplekser.

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