Name: Un método eficiente y rápido HPLC-QQQ-MS para la cuantificación de alcaloides tropanos en plantas medicinales (Video) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: Aprenda a cuantificar alcaloides tropanos en Anisodus tanguticus mediante HPLC-QQQ-MS. Este método analítico rápido y sensible supera las limitaciones de la cromatografía convencional para el control de calidad de plantas medicinales.

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<ol><li>Ji, P., Yang, X., Zhao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+metabolomics+in+quality+control+of+traditional+Chinese+medicines%3A+a+review%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Application of metabolomics in quality control of traditional Chinese medicines: a review.</a> Front Plant Sci. 15, 1463666(2024).</li>
<li>Li, S. -S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phytochemical+variation+among+the+traditional+Chinese+medicine+mu+dan+pi+from+Paeonia+suffruticosa+%28tree+peony%29%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Phytochemical variation among the traditional Chinese medicine mu dan pi from Paeonia suffruticosa (tree peony).</a> Phytochemistry. 146, 16-24 (2018).</li>
<li>Wang, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pinelliae+rhizoma+alleviated+acute+lung+injury+induced+by+lipopolysaccharide+via+suppressing+endoplasmic+reticulum+stress-mediated+NLRP3+inflammasome%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Pinelliae rhizoma alleviated acute lung injury induced by lipopolysaccharide via suppressing endoplasmic reticulum stress-mediated NLRP3 inflammasome.</a> Front Pharmacol. 13, 883865(2022).</li>
<li>Pughaih, T., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aPughaih%2C+T+%22Tropane+Alkaloids%3A+Sources%2C+Chemistry%2C+Pharmacology+and+Biotechnology%22">Tropane Alkaloids: Sources, Chemistry, Pharmacology and Biotechnology</a>. , Nature, Springer. Singapore. (2025).</li>
<li>Chen, C., Wang, B., Xiao, Y., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Geographical+origin+discrimination+of+Anisodus+tanguticus+%28Maxim.%29%3A+chemometrics+methods+applied+to+ICP-OES+analysis%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Geographical origin discrimination of Anisodus tanguticus (Maxim.): chemometrics methods applied to ICP-OES analysis.</a> Arab J Chem. 17 (5), 105730(2024).</li>
<li>Cardillo, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scopolamine%2C+anisodamine+and+hyoscyamine+production+by+Brugmansia+candida+hairy+root+cultures+in+bioreactors%5BTitle%5D)+AND+%22Process+Biochem%22%5BJournal%5D)">Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors.</a> Process Biochem. 45 (9), 1577-1581 (2010).</li>
<li>Yuan, B., Zheng, C., Teng, H., You, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+anisodamine%2C+and+scopolamine+in+plant+extract+by+nonaqueous+capillary+electrophoresis+coupled+with+electrochemiluminescence+and+electrochemistry+dual+detection%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection.</a> J Chromatogr A. 1217 (1), 171-174 (2010).</li>
<li>Zhang, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+scopolamine%2C+and+anisodamine+from+Hyoscyamus+niger+L.+in+rat+plasma+by+high-performance+liquid+chromatography+with+tandem+mass+spectrometry+and+its+application+to+a+pharmacokinetics+study%3A+liquid+chromatography%5BTitle%5D)+AND+%22J+Sep+Sci%22%5BJournal%5D)">Simultaneous determination of atropine, scopolamine, and anisodamine from Hyoscyamus niger L. in rat plasma by high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study: liquid chromatography.</a> J Sep Sci. 37 (19), 2664-2674 (2014).</li>
<li>Chen, C., Wang, B., Li, J., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+metabolites+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+to+determine+geographical+origins+and+network+pharmacology%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Multivariate statistical analysis of metabolites in Anisodus tanguticus (Maxim.) Pascher to determine geographical origins and network pharmacology.</a> Front Plant Sci. 13, 927336(2022).</li>
<li>Chen, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+tropane+alkaloids+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+from+different+regions+to+trace+geographical+origins%5BTitle%5D)+AND+%22Acta+Chromatogr%22%5BJournal%5D)">Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins.</a> Acta Chromatogr. 34 (4), 422-429 (2022).</li>
<li>Sawabe, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+determination+of+atropine+and+scopolamine+content+in+Scopolia+extract+powder+by+HPLC%5BTitle%5D)+AND+%22J+Nat+Med%22%5BJournal%5D)">Rapid determination of atropine and scopolamine content in Scopolia extract powder by HPLC.</a> J Nat Med. 65 (2), 395-399 (2011).</li>
<li>Baj, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+HPLC-QQQ-MS%2FMS+and+new+RP-HPLC-DAD+system+utilizing+the+chaotropic+effect+for+determination+of+nicotine+and+its+major+metabolites+cotinine%2Cand+trans-3%E2%80%B2-hydroxycotinine+in+human+plasma+samples%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Application of HPLC-QQQ-MS/MS and new RP-HPLC-DAD system utilizing the chaotropic effect for determination of nicotine and its major metabolites cotinine,and trans-3′-hydroxycotinine in human plasma samples.</a> Molecules. 27 (3), 682(2022).</li>
<li>Chen, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Qualitative+and+quantitative+analysis+of+the+major+constituents+in+Shexiang+tongxin+dropping+pill+by+HPLC-Q-TOF-MS%2FMS+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Qualitative and quantitative analysis of the major constituents in Shexiang tongxin dropping pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS.</a> Molecules. 20 (10), 18597-18619 (2015).</li>
<li>Huang, Y. -F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+twenty-nine+active+compounds+in+Fuzhengjiedu+granules+by+HPLC-QQQ-MS%2FMS%5BTitle%5D)+AND+%22Heliyon%22%5BJournal%5D)">Simultaneous determination of twenty-nine active compounds in Fuzhengjiedu granules by HPLC-QQQ-MS/MS.</a> Heliyon. 9 (2), e13675(2023).</li>
<li>Zhang, K. -X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+quality+control+method+for+prescriptions+of+Polygonum+capitatum+through+simultaneous+determination+of+nine+major+constituents+by+HPLC+coupled+with+triple+quadruple+mass+spectrometry%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Improved quality control method for prescriptions of Polygonum capitatum through simultaneous determination of nine major constituents by HPLC coupled with triple quadruple mass spectrometry.</a> Molecules. 18 (10), 11824-11835 (2013).</li>
<li>Ding, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comprehensive+chemical+profiling+and+quantification+of+Shexiang+xintongning+tablets+by+integrating+liquid+chromatography-mass+spectrometry+and+gas+chromatography-mass+spectrometry%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Comprehensive chemical profiling and quantification of Shexiang xintongning tablets by integrating liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry.</a> Arab J Chem. 16 (3), 104527(2023).</li>
<li>Zhu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+QuEChERS+method+coupled+to+HPLC-QQQ-MS%2FMS+for+the+determination+of+25+banned+pesticide+residues+in+ethnic+medicines%5BTitle%5D)+AND+%22Res+Sq%22%5BJournal%5D)">The QuEChERS method coupled to HPLC-QQQ-MS/MS for the determination of 25 banned pesticide residues in ethnic medicines.</a> Res Sq. , (2022).</li>
<li>Cinelli, M. A., Cline, J., Watson, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applications+of+liquid+chromatography-tandem+mass+spectrometry+in+natural+products+research%3A+Tropane+alkaloids+as+a+case+study%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Applications of liquid chromatography-tandem mass spectrometry in natural products research: Tropane alkaloids as a case study.</a> J Vis Exp. (205), e66620(2024).</li>
<li>Pang, Y., Huang, Y. -K., Li, F., Yang, F. -Q., Xia, Z. -N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+screening+and+evaluation+of+antioxidants+in+alkaloid+natural+products+by+capillary+electrophoresis+with+chemiluminescence+detection%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Rapid screening and evaluation of antioxidants in alkaloid natural products by capillary electrophoresis with chemiluminescence detection.</a> Anal Methods. 8 (35), 6545-6553 (2016).</li>
<li>Voeten, R. L. C., Ventouri, I. K., Haselberg, R., Somsen, G. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Capillary+electrophoresis%3Atrends+and+recent+advances%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Capillary electrophoresis:trends and recent advances.</a> Anal Chem. 90 (3), 1464-1481 (2018).</li>
<li>Pellati, F., Benvenuti, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromatographic+and+electrophoretic+methods+for+the+analysis+of+phenetylamine+alkaloids+in+Citrus+aurantium%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Chromatographic and electrophoretic methods for the analysis of phenetylamine alkaloids in Citrus aurantium.</a> J Chromatogr A. 1161 (1), 71-88 (2007).</li>
<li>Cirlini, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+sensitive+UHPLC-ESI-MS%2FMS+method+for+the+determination+of+tropane+alkaloids+in+herbal+teas+and+extracts%5BTitle%5D)+AND+%22Food+Control%22%5BJournal%5D)">A sensitive UHPLC-ESI-MS/MS method for the determination of tropane alkaloids in herbal teas and extracts.</a> Food Control. 105, 285-291 (2019).</li>
<li>Wojakowska, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structural+analysis+and+profiling+of+phenolic+secondary+metabolites+of+Mexican+lupine+species+using+LC-MS+techniques%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Structural analysis and profiling of phenolic secondary metabolites of Mexican lupine species using LC-MS techniques.</a> Phytochemistry. 92, 71-86 (2013).</li>
<li>Shimizu, T., Watanabe, M., Fernie, A. R., Tohge, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+LC-MS+analysis+for+plant+secondary+metabolites%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Targeted LC-MS analysis for plant secondary metabolites.</a> Methods Mol Biol. 1778, 171-181 (2018).</li>
<li>Mennickent, S., De Diego, M. Analytical Method Validation as the First Step in Drug Quality Control. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMennickent%2C+S+%22Quality+Management+and+Quality+Control+-+New+Trends+and+Developments%22">Quality Management and Quality Control - New Trends and Developments</a>. , IntechOpen. London, UK. (2019).</li>
<li>Yang, S. H., Wang, J., Zhang, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+a+two-dimensional+liquid+chromatography+method+for+quality+control+testing+of+pharmaceutical+materials%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Validation of a two-dimensional liquid chromatography method for quality control testing of pharmaceutical materials.</a> J Chromatogr A. 1492, 89-97 (2017).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HPLC+with+chiral+stationary+phase+for+separation+and+kinetics+study+of+aspartic+acid+epimerization+in+peroxiredoxin+2+active+site+peptide%5BTitle%5D)+AND+%22J+Pharm+Biomed+Anal%22%5BJournal%5D)">HPLC with chiral stationary phase for separation and kinetics study of aspartic acid epimerization in peroxiredoxin 2 active site peptide.</a> J Pharm Biomed Anal. 247, 116247(2024).</li>
<li>Lu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolite+measurement%3A+pittfalls+to+avoid+and+practices+to+follow%5BTitle%5D)+AND+%22Annu+Rev+Biochem%22%5BJournal%5D)">Metabolite measurement: pittfalls to avoid and practices to follow.</a> Annu Rev Biochem. 86, 277-304 (2017).</li>
<li>Leopold, J., Popkova, Y., Engel, K. M., Schiller, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+developments+of+useful+MALDI+matrices+for+the+mass+spectrometric+characterization+of+lipids%5BTitle%5D)+AND+%22Biomolecules%22%5BJournal%5D)">Recent developments of useful MALDI matrices for the mass spectrometric characterization of lipids.</a> Biomolecules. 8 (4), 173(2018).</li>
<li>Parasuraman, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+overview+of+liquid+chromatography-mass+spectroscopy+instrumentation%5BTitle%5D)+AND+%22Pharm+Methods%22%5BJournal%5D)">An overview of liquid chromatography-mass spectroscopy instrumentation.</a> Pharm Methods. 5 (2), 47-55 (2014).</li>
<li>Alanazi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+liquid+chromatography%E2%80%93mass+spectrometry+%28LC%E2%80%93MS%29+applications+in+biological+and+applied+sciences%5BTitle%5D)+AND+%22Anal+Sci+Adv%22%5BJournal%5D)">Recent advances in liquid chromatography–mass spectrometry (LC–MS) applications in biological and applied sciences.</a> Anal Sci Adv. 6 (1), e70024(2025).</li>
<li>Cortese, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Compensate+for+or+minimize+matrix+effects%3F+Strategies+for+overcoming+matrix+effects+in+liquid+chromatography-mass+spectrometry+technique%3A+A+tutorial+review%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Compensate for or minimize matrix effects? Strategies for overcoming matrix effects in liquid chromatography-mass spectrometry technique: A tutorial review.</a> Molecules. 25 (13), 30-47 (2020).</li>
<li>Wang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+interplay+between+herbal+medicines+and+gut+microbiota+in+metabolic+diseases%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">The interplay between herbal medicines and gut microbiota in metabolic diseases.</a> Front Pharmacol. 14, 1105405(2023).</li>
<li>Musharraf, S. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Screening+of+inhibitors+of+angiotensin-converting+enzyme+%28ACE%29+employing+high+performance+liquid+chromatography-electrospray+ionization+triple+quadrupole+mass+spectrometry+%28HPLC-ESI-QqQ-MS%29%5BTitle%5D)+AND+%22Eur+J+Pharm+Sci%22%5BJournal%5D)">Screening of inhibitors of angiotensin-converting enzyme (ACE) employing high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (HPLC-ESI-QqQ-MS).</a> Eur J Pharm Sci. 101, 182-188 (2017).</li>
<li>Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Integrating+qualitative+and+quantitative+assessments+of+Yougui+pill%2C+an+effective+traditional+Chinese+medicine%2C+by+HPLC-LTQ-orbitrap-MSn+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-orbitrap-MSn and UPLC-QqQ-MS/MS.</a> Anal Methods. 9 (23), 3485-3496 (2017).</li>
<li>Parks, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redirecting+tropane+alkaloid+metabolism+reveals+pyrrolidine+alkaloid+diversity+in+Atropa+belladonna%5BTitle%5D)+AND+%22New+Phytol%22%5BJournal%5D)">Redirecting tropane alkaloid metabolism reveals pyrrolidine alkaloid diversity in Atropa belladonna.</a> New Phytol. 237 (5), 1810-1825 (2023).</li>
<li>Yan, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Triterpenoid+saponins+profiling+by+adducts-targeted+neutral+loss+triggered+enhanced+resolution+and+product+ion+scanning+using+triple+quadrupole+linear+ion+trap+mass+spectrometry%5BTitle%5D)+AND+%22Anal+Chim+Acta%22%5BJournal%5D)">Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry.</a> Anal Chim Acta. 819, 56-64 (2014).</li>
<li>Sosa-Ferrera, Z., Mahugo-Santana, C., Santana-Rodríguez, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analytical+methodologies+for+the+determination+of+endocrine+disrupting+compounds+in+biological+and+environmental+samples%5BTitle%5D)+AND+%22Biomed+Res+Int%22%5BJournal%5D)">Analytical methodologies for the determination of endocrine disrupting compounds in biological and environmental samples.</a> Biomed Res Int. 2013, 674838(2013).</li>
<li>Heyde, B. J., Barthel, A., Siemens, J., Mulder, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+fast+and+robust+method+for+the+extraction+and+analysis+of+quaternary+alkyl+ammonium+compounds+from+soil+and+sewage+sludge%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">A fast and robust method for the extraction and analysis of quaternary alkyl ammonium compounds from soil and sewage sludge.</a> PLoS One. 15 (8), e0237020(2020).</li>
</ol>

<table><tbody><tr><td>Agilent MassHunter software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 column</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 reversed-phase column, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>Anisodamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Formic Acid</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>freeze dryer</td><td>EYELA, Tokyo, Japan</td><td>FDU-2110</td><td></td></tr><tr><td>High-speed universal grinder</td><td>Taisite Instrument Co., Ltd., Tianjin, China</td><td>6010210100</td><td>FW-100,24,000 rpm, 100 g capacity, 80 mesh</td></tr><tr><td>Hyoscyamine</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Microporous Membrane 0.22&mu;m</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 water system</td><td>MilliporeSigma, Burlington, MA, USA</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Scopolamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

un método eficiente y rápido hplc-qqq-ms para la cuantificación de alcaloides tropanos en plantas medicinales

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

Separación cromatográfica e identificación de TA HPLC-QQQ-MS Un cromatograma típico de MRM obtenido a partir del análisis de una mezcla estándar y un extracto de raíz de A. tanguticus se presenta en las Figuras 3 y 4. Bajo las condiciones optimizadas del HPLC, los tres alcaloides de tropano objetivo: hiosciamina, anisodamina y escopolamina, se separaron con éxito en menos de 20 minutos, demostrando l...

Un método eficiente y rápido HPLC-QQQ-MS para la cuantificación de alcaloides tropanos en plantas medicinales

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

an-efficient-rapid-hplc-qqq-ms-method-for-quantitation-tropane

Research

Investigación

JoVE Journal

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Presentamos un método para la anotación y clasificación de alcaloides tropanos basada en espectrometría de masas (MS) rápida/espectrometría de masas, útil tanto para la dereplicación preliminar de muestras que contienen tropano como para el descubrimiento de nuevos alcaloides para el aislamiento.

Aplicaciones de la espectrometría de masas en tándem de cromatografía líquida en la investigación de productos naturales: alcaloides tropanos como estudio de caso

Este protocolo presenta un método validado de cromatografía líquida-movilidad iónica-espectrometría de masas de alta resolución para determinar la presencia de alcaloides del cornezuelo de centeno en los alimentos en cumplimiento del Reglamento (UE) 2023/915 de la Comisión recientemente publicado.

Detección de alcaloides de cornezuelo de centeno regulados en matrices alimentarias por cromatografía líquida-espectrometría de movilidad iónica atrapada-espectrometría de masas de tiempo de vuelo

Este protocolo detalla los pasos importantes que se requieren para la bioconjugación de una cisteína que contiene proteína a una maleimida, incluyendo la purificación de reactivos, condiciones de reacción, purificación y caracterización bioconjugado bioconjugado.

Síntesis de Proteínas bioconjugados<em&gt; vía</em&gt; Química cisteína-maleimida

Describimos la polimerización por inserción catalítica del ácido 5-norborneno-2-carboxílico y 5-vinil-2-norborneno para formar polímeros funcionales con una temperatura de transición vítrea muy alta.

Un protocolo simple y eficaz para la inserción catalítico de polimerización norbornenos funcionales

Se describe un procedimiento detallado para la síntesis de una azida marcado con I125 y el radiomarcado de dibenzocyclooctyne (DBCO) -grupo-conjugados, las nanopartículas de oro de 13 nm de tamaño utilizando una reacción clic libre de cobre.

Un protocolo optimizado para el radiomarcaje eficaz de nanopartículas de oro mediante el uso de una<sup&gt; 125</sup&gt; Azida grupo prostético marcado con I

Aprenda a cuantificar alcaloides tropanos en Anisodus tanguticus mediante HPLC-QQQ-MS. Este método analítico rápido y sensible supera las limitaciones de la cromatografía convencional para el control de calidad de plantas medicinales.

Un método eficiente y rápido HPLC-QQQ-MS para la cuantificación de alcaloides tropanos en plantas medicinales

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