Sackler School of Graduate Biomedical Sciences 1 article published in JoVE Immunology and Infection Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers Alyssa C. Fasciano1, Sarah E. Blutt2, Mary K. Estes2, Joan Mecsas3 1Program in Immunology, Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology, Baylor College of Medicine, 3Department of Molecular Biology and Microbiology, Tufts University This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.