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Immunology and Infection
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Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Article DOI: 10.3791/2383-v • 17:30 min • January 15th, 2011
January 15th, 2011 •
1, 1, 1, 2
1Department of Systems Biology, Danish Technical University, 2Department of Biology, University of Copenhagen

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Protocol describing the application of a flow cell system for growing and analyzing microbial biofilms for Confocal Laser Scanning Microscopy (CLSM).

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Pseudomonas Aeruginosa Saccharomyces Cerevisiae Biofilm Flow Cells Microbial Communities Altered Physiological Properties Pathological Properties Transparent Substratum Non-destructive Examination Real-time Studies Flow Cell Model System Reproducibility Growth Chamber Nutrients Oxygen Supply Peristaltic Pump Waste Container Continuous Supply Of Nutrients Administration Of Antibiotics Minimal Disturbance Shear Stress Bubble Trapping Device Confocal Laser Scanning Microscopy (CLSM) 3D Information
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