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Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish

Article DOI: 10.3791/2790-v • 08:01 min • July 14th, 2011
July 14th, 2011 •
1, 1, 2
1Department of Molecular Pathology, Massachusetts General Hospital, Harvard Medical School, 2Department of Molecular Pathology, Massachusetts General Hospital Cancer Center, Harvard Stem Cell Institute

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Limiting dilution cell transplantation assays are used to determine the frequency of tumor-propagating cells. This protocol describes a method for generating syngeneic zebrafish that develop fluorescently-labeled leukemia and details how to isolate and transplant these leukemia cells at limiting dilution into the peritoneal cavity of adult zebrafish.

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Quantifying Frequency Tumor-propagating Cells Limiting Dilution Cell Transplantation Syngeneic Zebrafish Self-renewing Cancer Cells Tumor Growth Tumor-initiating Cells Destruction Tumor Progression Relapse Patient Prognosis Determining Frequency Transplantation Assay Recipient Animals Increasing Doses Develop Tumors Original Tumor Sample Large Number Of Animals Limiting Dilution Experiment Mice Costly Zebrafish As A Cancer Model Genetic Manipulation Large Scale Experiments Cancer Types Modeled In Zebrafish
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