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Developmental Biology

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JoVE Journal Developmental Biology
Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
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Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

Article DOI: 10.3791/53635-v • 10:09 min • May 25th, 2016
May 25th, 2016 •
1, 1, 1, *2, *1
1Cell Biology, UMC Groningen, 2Clinical Genetics, Erasmus MC Rotterdam
* These authors contributed equally

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résumé
Automatic Translation

Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution EM. Here we describe a universal method for nanotomy applied to investigate the zebrafish larval brain in health and upon non-invasive brain injury.

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Large-scale Scanning Transmission Electron Microscopy Nanotomy Zebrafish Brain Macromolecular Level Tissue Level Degenerating Brain Neurodegeneration Type 1 Diabetes Research Unbiased Information Quantitation Open-access Data Sharing Immune Maintenance Microglia Disease Models Cell Culture Mouse Tissues Human Tissues Overwhelming Data Fixing Zebrafish Larvae Osmium Tetroxide Potassium Ferrocyanide Sodium Cacodylate Double Distilled Water Ethanol Embedding
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