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Developmental Biology

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JoVE Journal Developmental Biology
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

Article DOI: 10.3791/53750-v • 07:44 min • March 18th, 2016
March 18th, 2016 •
1,3, 1, 1,2,3
1Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, 2Department of Medicine, University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry

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Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

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Epicardial Outgrowth Culture Assay Ex Vivo Assessment Epicardial-derived Cell Migration Primary Cultures Epithelial To Mesenchymal Transitions Functional Migration Epicardial Derived Progenitor Cells Cardiac Regenerative Medicine Collagen Coated Chamber Slides Medium A Isolation Of Primary Epicardial Cells Mouse Embryos Thoracic Wall Chest Cavity Outflow Tract Collagen Coated Slide Incubation
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