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Immunology and Infection

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Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Article DOI: 10.3791/53933-v • 08:47 min • May 8th, 2016
May 8th, 2016 •
*1, *2, 2, 2, 2, 1, 2
1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME
* These authors contributed equally

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This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.

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Experimental Autoimmune Encephalomyelitis EAE Model Immune Cells Central Nervous System Multiple Sclerosis Demyelination Drug Therapy Stress-free Environment Pertussis Toxin Lymph Nodes Spinal Cord Myelin Oligodendrocyte Glycoprotein Complete Freund's Adjuvant Mycobacterium T
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