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A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side

Article DOI: 10.3791/54061-v • 09:58 min • May 6th, 2016
May 6th, 2016 •
1, 1, 1, 2, 1, 1, 1
1Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 2Department of NDU Life Sciences, Nippon Dental University

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résumé
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The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.

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Choroid Plexus Epithelial Cell-based Model Human Blood-cerebrospinal Fluid Barrier Bacterial Infection Basal-lateral Side Infectious Diseases Choroid Plexus Epithelium Pathogens Transmigration Immune Cells Tumor Cells HIBCCP Cells Growth Area Cell Culture Filter Inserts Pre-warmed Medium Subculture Medium Supplement
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