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Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
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Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Article DOI: 10.3791/55002-v • 10:13 min • December 12th, 2016
December 12th, 2016 •
1,2, 2,3, 1,2, 3,4, 2,3, 4, 5, 5, 1,2,3
1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

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résumé
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Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.

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Drosophila Longitudinal Imaging Heart Function In Vivo Optical Coherence Microscopy OCM Drosophila Cardiac Development Noninvasive Imaging Spatial Resolution Temporal Resolution Genetic Similarities Cardiac Disease Vertebrates Optogenetic Pacing Pacemaker Function Animal Models Reproductive Parental Flies Eggs Larva Mounting Double-sided Tape
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