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Høy presisjon FRET ved enkeltmolekylivå for biomolekylstrukturbestemmelse

Article DOI: 10.3791/55623-v • 11:24 min • May 13th, 2017
May 13th, 2017 •
1, 2, 2, 3, 3, 2
1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center

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En protokol for høy presisjon FRET-eksperimenter på enkeltmolekylnivå presenteres her. I tillegg kan denne metoden brukes til å identifisere tre konformasjonelle tilstande i det ligandbindende domenet til N-metyl-D-aspartat (NMDA) reseptoren. Fastsettelse av nøyaktige avstander er det første skrittet mot bygging av strukturelle modeller basert på FRET-eksperimenter.

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Biochemistry FRET enkeltmolekyl interdye avstand DNA fluorofor multiparameter fluorescens deteksjon pulserende interleaved excitation protein merking
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