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JoVE Journal Biochemistry
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
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A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Article DOI: 10.3791/55923-v • 12:06 min • October 1st, 2017
October 1st, 2017 •
1,2, 1,2
1Broad Institute, Massachusetts Institute of Technology and Harvard Medical School, 2Dept. of Biological Engineering, Massachusetts Institute of Technology

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This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.

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Single Molecule Flow Stretching Assay Transport Of Molecules Along DNA Biotin-labeled Lambda-DNA PEG Functionalized Coverslips High Throughput Flow Cell Polydimethylsiloxane (PDMS) Slab Inlet And Outlet Holes Time-lapse Total Internal Reflection Fluorescence (TIRF) Imaging Single Particle Tracking Software Coverslip Preparation Higher Throughput Capacity Reduced Hands-on Time Unsupervised Data Analysis Dr. Kan Xiong
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