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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
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Journal JoVE Biologie
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

DOI:
10.3791/57385-v

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08:26 min

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May 01, 2018

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, , , ,
1Department of Entomology,National Taiwan University, 2Biology Centre, Institute of Entomology,Czech Academy of Sciences, 3Faculty of Science,University of South Bohemia, 4Institute of Evolutionary Biology, CSIC-UPF

Chapitres

  • 00:04Titre
  • 00:50Collection of Extracellular Enzymes from Hemolymph
  • 02:13Collection of Extracellular Enzymes from Midgut Juice
  • 03:31dsRNA Degradation Assay
  • 05:31Oral Administration of dsRNA
  • 06:42Results: Knock Down of Tubulin Expression in the Midgut
  • 07:38Conclusion

Summary

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Traduction automatique

This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.

Tags

RNA InterferenceDouble-stranded RNALiposome CarriersCockroachesPest ControlGene SilencingHemolymph CollectionMidgut JuiceInsect Saline BufferProtein Quantification

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