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Biochemistry

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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

Article DOI: 10.3791/58139-v • 08:10 min • July 28th, 2018
July 28th, 2018 •
*1, *1, 1
1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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résumé
Automatic Translation

We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.

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In Vitro Reconstitution Self-Organizing Protein Patterns Supported Lipid Bilayers Cell Organization Membranes Geometric Cues Pattern Formation Protein Concentrations Multilamellar Vesicles Freeze-thaw Lipids Lipid Extruder Min Buffer Glass Coverslips Sulfuric Acid Hydrogen Peroxide
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