JoVE Journal
Immunology and Infection
Immunology and Infection
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
We describe a protocol for a three-dimensional co-culture model of infected airways, using CFBE41o- cells, THP-1 macrophages, and Pseudomonas aeruginosa, established at the air-liquid interface. This model provides a new platform to simultaneously test antibiotic efficacy, epithelial barrier function, and inflammatory markers.