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Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Article DOI: 10.3791/61226-v • 08:06 min • July 8th, 2020
July 8th, 2020 •
1, 1, 2, 1, 3, 4, 1,5, 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Shijiazhuang Shimen Experimental School, 3Qilu Children's Hospital of Shandong University, 4Qilu Hospital of Shandong University, 5Shenzhen Research Institute of Shandong University

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This paper reports that the addition of Y-27632 to TIVA medium can significantly increase the yield of melanocytes from adult skin tissues.

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Y-27632 Human Melanocytes Adult Skin Tissues Cell Isolation Cell Culture Conventional Methods Primary Melanocytes Cells Circumcision Surgery Human Research Ethics Committee Reagents Sterile Instruments Washes Solution 75% Ethanol Surgical Blade Fat Layer Dermis Side Down Space Solution Epidermis Washing Solution Trypsin Neutralization Solution
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