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Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Article DOI: 10.3791/61805-v • 08:32 min • October 2nd, 2020
October 2nd, 2020 •
1, 1,2,3, 1,2, 1, 1, 1, 1,2,4
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, 2Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, 3Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, 4Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University

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We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ.

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Computer-based Image Analysis Lung Metastasis Breast Cancer Model Quantification Precision Efficiency Preclinical Research Breast Cancer Therapies Metastatic Burden Collagenase Mixture Elastase Tissue Sample HBSS Lung Pieces Incubate Centrifuge Tube Tissue Culture Plate
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