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Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Article DOI: 10.3791/62373-v • 12:08 min • June 19th, 2021
June 19th, 2021 •
1,2, 1, 1
1Champalimaud Centre for the Unknown, Champalimaud Foundation, 2Instituto Gulbenkian de Ciência

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Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.

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Zebrafish Xenografts Cancer Research Human Tumor Cells Larva Model Cancer Hallmarks Proliferation Angiogenesis Metastasis Tumor Microenvironment Confocal Microscope Light Sheet Microscope Single Cell Resolution Cancer Biology Response To Anti-cancer Therapies Chemotherapy Radiotherapy Targeted Therapies Antibodies Patient-derived Cells Surgical Sample Biopsies Zebrafish Avatars
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