גישת עיבוד מקביל רב היעד לקביעת גנטי למבנה של פולימראז שפעת PB2 מקטע

2:20

Construct Design

4:07

Polymerase Incomplete Primer Extension (PIPE) Cloning

6:14

Expression Optimization

8:21

Large Scale Fermentation

9:40

Protein Purification - Cell Lysis

10:54

Protein Purification - Affinity Chromatography

13:04

Protein Purification - Affinity Tag Cleavage

14:47

Protein Purification - Size-exclusion Chromatography (SEC)

15:42

Protein Crystallization

17:03

Crystal Harvesting

18:15

Crystal Screening/Data Collection

19:27

Results: 3D Structure Determination of Influenza Virus PB2

21:05

Conclusion